Genetically modified flax differs in response to Fusarium infection
نویسندگان
چکیده
Flax (Linum usitatissimum L.) is an annual plant cultivated mainly for its fibres. Although the whole genus contains about 230 species, only L. usitatissimum retains an important commercial use, serving as a fibre source for the textile industry. Flax fibres are less elastic than cotton fibres, and thus their use in textile production is more limited. However, the climate and soil quality in central and eastern Europe are extraordinary for flax cultivation. Thus, in Poland, some effort has been put into improving the quality of flax fibres in a recent years. Many determinants of different natures affect fibre quality. The chemical composition of the fibre, the efficiency of the stem retting, and the plant’s resistance to pathogen infection are among the most important. Multidirectional, genetically modifications of flax were performed to improve fibre quality, retting efficiency and resistance to Fusarium infection. To accomplish this, generation of transgenic flax plants with PHB (poly-βhydroxybutyrate) synthesis, increased polygalacturonase (PGI ), rhamnogalacturonase (RHA), β-glucanase activities and silenced CAD (cinnamyl alcohol dehydrogenase) gene was performed. What is interesting all of those modifications resulted in different response to pathogen attack. Since fungi from Fusarium genus are main flax pathogen, the resistance of all created transgenic flax plants against F. oxysporum and F. culmorum was investigated and compared to non-transformants. Flax seedlings were analyzed by the mycelium method. A different response to pathogenic infection for various transgenic flax plants was observed i.e. CAD-deficient plants exhibited over two-fold lower resistance to Fusarium infection than nontransformed plants, while flax plants enriched with PHB showed about two-fold higher resistance to the same, investigated pathogen. Analysis of metabolites, which interfere with plant defense in various transgenic plants was performed to find out the reason for this. P14.24
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